Alyssa Rosenbloom, PhD

NRSA Postdoctoral Fellow, co-advised by David V. Schaffer
University of California, Berkeley

Ph.D. Molecular and Cell Biology, 2012

University of California, Berkeley

B.S. Genetics and Biochemistry, 2006
Texas A&M

Office Location: 278 Stanley Hall
Office Telephone: 510-642-4923

Neural stem cells (NSCs) reside in signaling environments that present a dynamic repertoire of instructive signals, and it is becoming clear that fate decisions are regulated not simply by the presence/absence but by temporal and intensity modulation of signaling factors and their downstream cascades. Because of the highly combinatorial nature of this problem, however, knowledge of how these signals cooperate to regulate cell function is limited due to the current low-throughput experimental systems and the lack of developed reporters for real-time analysis. I am developing a library of genetic transcription activation multiple reporter cassettes which will be stably integrated into the NSC genome to allow for multiplexed observations of cellular signaling and gene expression events that drive different cell fates. To obtain high-throughput data sets, NSCs are seeded into 3D synthetic ECM microenvironments onto micropillars and media solutions are deposited in parallel into a complementary microwells of a then sandwiched chip with the in-house automated high-throughput micro-arrayer system. By combining stably integrated genetic fluorescent reporter cassettes for real-time monitoring of transcriptional activities and a high-throughput cellular micro-array approach involving micro-spotting of cell suspensions in an artificial ECM to create 3D niches, our model system allows for the exploration of the combinatorial influence of dynamic (dosage and temporal) biochemical signals on NSC fate decisions. 

Hobbies, I got’em. :D